DOT 5-Panel Drug Test (Urine Only) Purpose of the Test A core DOT requirement for identifying the presence of five major drug classes through urine collection. Drugs Detected Tests for THC, Cocaine, Amphetamines, Opiates, and PCP—substances strictly regulated in DOT guidelines. Where It’s Required Mandatory in the transportation, aviation, pipeline, maritime, and railroad industries. Secure Collection Process Samples are collected following DOT-certified procedures with full chain-of-custody documentation. Peace of Mind Ensures your business stays compliant with federal safety rules and reduces workplace liability.